SCIN 130 AMU Lab 7 CSI Wildlife Case 1 Introduction to Biology American Military University assistance is available on Domyclass
Step 1: Read and complete the Lesson for the week – both the Lecture and Lab portion found in the APUS online classroom! The information you acquire in this Lab will be included in your quiz for the week – so make sure you have it completed prior to taking the quiz!
Step 2: Complete all the activities in this lab instruction packet: SCIN 130 Lab 7: CSI Wildlife, Case 1. Work through the instruction packet step by step. Record your results directly in the worksheet as you progress through the questions.
Step 3: You should save a copy of your completed lab with your name and lab # in the title. For example, if you are Felicetti and this is Lab 7, you would title your document: FelicettiLab7.doc. You are then to add this as an attachment to the quiz for the week!
Please note the following:
For any sections that request that you “take notes”, the notes should be in your own words summarizing information learned. You should not copy and paste information from the Internet including media and resources accessed in this lab. Directly copying and pasting information is considered plagiarism in this course.
When taking screenshots save with file name that has your last name, the lab number and the screenshot number. For example, for the first screenshot in Lab 7 if your last name was Felicetti the file name would be FelicettiLab7Shot1.jpeg. It is important to follow this image labeling structure. Images submitted without proper labeling will be graded as a zero.
This lab instruction packet will be submitted as an attachment with the quiz for the week when completed. Again, save the file with your name and the lab number as a Word document. For example, if this was Lab 7 and your last name was Felicetti the file name would be FelicettiLab7.docx. It is important to follow this file labeling structure. Files submitted without proper labeling are subject to a score of zero.
SCIN 130 Lab 7: CSI Wildlife, Case 1
Be sure to read the general instructions from the Lessons portion of the class prior to completing this packet.
Remember, you are to upload this packet with your quiz for the week!
The scenarios investigated are based on the recently published literature: Wasser, S. K., Brown, L., Mailand, C., Mondol, S., Clark, W., Laurie, C., & Weir, B. S. (2015). Genetic assignment of large seizures of elephant ivory reveals Africa’s major poaching hotspots. Science, 349(6243), 84–87. The underlying data are available on the Dryad Digital Repository: http://dx.doi.org/10.5061/dryad.435p4.
Remember, DNA is made up of nucleotides and an allele is an
alternative form of a gene which may be from mutation, but is found on the same
place in a chromosome in individuals and functions similarly. If you are
unfamiliar with these terms, make sure to review them in your book prior to
completing the lab.
Specific Lab Instructions
Part 1: The Introduction
- Read the instructions on the home page. Then, watch the opening video from the CSI Wildlife Introduction.
- What is a keystone species?
- Dr. Wasser states that approximately 50,000 African elephants are killed each year. According to the video, it is estimated that there are around 470,000 African elephants. If these numbers are correct, approximately what percentage of African elephants are killed each year? (Show your work.)
- In one or two sentences, summarize Dr. Wasser’s research and how it is being used to conserve elephants.
Part 2: Case One
- Watch the crime scene video on the first slide of Case One. Explain the goal of the case.
- Look at the map provided; in what type of location are the majority of African elephants located?
- Proceed to the How DNA Profiling Works section.
- What does “STR” stand for and how are they important for identification?
- Look at the gel on the screen. What do the bands on the agarose gel represent?
- What is the purpose of the DNA ladder on the agarose gel?
- DNA profiling is also called DNA fingerprinting. A common misconception about DNA fingerprinting is that the analysis has to do with actual fingerprints. Explain one similarity and one difference between a human being’s pattern of bands on an electrophoresis gel and a human fingerprint.
- Click on Technique.
- List three sources to obtain elephant DNA for analysis.
- Watch the animation on the polymerase chain reaction under Technique. What is the purpose of heating the DNA strand? What is the purpose of cooling the DNA strand?
- What is the relationship between the size of a DNA fragment and the distance it migrates in the gel?
- Why does DNA migrate to the positive electrode?
- Run the gel in the Technique section by pressing the Start button. Which elephant (left or right) has both the largest and smallest fragments? Approximately what size is the largest fragment (bp)? Smallest? .
- Proceed to the Application section and look at the gel.
- For Marker C, are the two elephants in the gel on the left homozygous or heterozygous? How do you know?
- Read the Review section, and make sure you can answer the questions.
- Go to Finding a Match
- Click on the “+” next to each marker. Compare the bands in the agarose gel from the unidentified elephant and the known elephants. Which elephant (there are two pages of them) matches the unidentified elephant?
- Watch the video on the “Case Solved” slide.
- Name two properties of a good marker and explain why good markers are important.
Adapted from: Click and Learn “CSI Wildlife” (2016). CSI Wildlife Explorer Worksheet. HHMI Biointeractive Teaching Materials.